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Toxicity Reference Database (ToxRefDB) contains in vivo study data from over 5900 guideline or guideline-like studies for over 1100 chemicals. By employing a controlled vocabulary for enhanced data quality, ToxRefDB (v2.1, released August 2022) serves as a resource for study design, quantitative dose response, and endpoint and effect controlled vocabulary linked to the required, recommended, or triggered measurements indicated by corresponding guideline specifications. The database can aid in the validation of in vitro high-throughput screening of chemicals and support retrospective and predictive toxicology applications.